Room Temperature Tissue Homogenizer Laboratory Sample Grinding Machine
Room temperature tissue homogenizer is a laboratory device used to break down tissues, cells, or other biological samples into a uniform mixture (homogenate) at ambient room temperature.
Cat. No. :
YB-HVBrand :
YanbiotechProduct Origin :
ChinaSpec. :
Room TemperatureProduct Introduction
Room Temperature Tissue Homogenizer is essential for extracting proteins, RNA, DNA, and other cellular components for further analysis, such as Western blotting, PCR, or enzyme assays.
These homogenizers can vary in terms of their design and operation, but they typically use mechanical force (e.g., shearing, grinding, or ultrasonic waves) to disrupt the tissue.
Tissue homogenizer utilizes a vertical shock system with high-speed reciprocating motion to make the frozen samples in the grinding tube collide with the grinding beads, The resulting grinding shear force and impact force completely crush the tissues, making it a specialized device for rapid one-time processing of multiple samples in research institutions,universities, agricultural colleges, biomedical and food testing fields.
Product Specifications
Product Name | Room Temperature |
Cat. No. | YB-HV |
Screen Size | 5 Inches |
Operation Time | Operation time:0-9999s, Pause time: 0-999s, Operation cycles:1-99s |
Power | 200-240VAC(110Vavailable),50-60Hz.240W |
Operating Location | Indoor |
Room Temperature | 5℃-40℃ |
Dimension | 294*452*376mm |
Weight | 26.2kg |
Key Features
Linear Guide Rail: stable and durable, capable of continuous grinding 24 hours a day.
Process Up to 24 Samples Simultaneously
Various Adapters Available
Compact body and beautiful appearance
Protein Extraction
Product Name | Grinding Beads | Grinding Time | Pause Time | Frequency |
Liver and Spleen | 4mm*1 3mm*1 |
30s | 15s | 60Hz |
Muscle | 4mm*2 3mm*2 |
45s | 15s | 60Hz |
Skin | 4mm*2 3mm*3 |
60s | 20s | 70Hz |
Blood Vessel | 4mm*2 3mm*2 |
60s | 15s | 70Hz |
Aorta | 4mm*2 3mm*3 |
60s | 15s | 70Hz |
Soft Bone | 4mm*2 3mm*3 |
60s | 20s | 70Hz |
Plant Leaf | 4mm*1 3mm*2 |
45s | 15s | 60Hz |
Plant Stem | 4mm*1 3mm*2 |
60s | 15s | 60Hz |
Hair | 4mm*2 3mm*3 |
90s | 20s | 70Hz |
Hard Bone | 4mm*2 3mm*4 |
90s | 30s | 70Hz |
For RNA extraction,the grinding time should be appropriately extended compared to protein extraction.Add RNAextraction buffer during grinding.The specific grinding time depends on the amount and type of tissue beingground.Samples that are difficult to grind can be repeatedly ground 2-3 times,and the specific situation can beadjusted appropriately based on the experiment.
Grinding of strong and tough tissues
For samples that are difficult to grind (such as soft bones,hair,etc.),a2ml grinding tube can be used.The sample should be cut into small pieces of about 1-2mm,and then add an appropriate amount ofgrinding beads.After tightly closing the grinding tube,put it into liquid nitrogen and freeze for 2-3min.Do not add extraction solution and grind for 60 seconds at a speed of 7m/s.Then add extractionsolution to the grinding tube and repeat the grinding process.If the grinding effect is not satisfactory,the sample can be frozen again and the above experimental steps can be repeated.
Applications
FAQ
Q. How many samples can be grinded in one time?
A. 24 samples can be processed with standard in 1 minute, efficiently and effectively completing sample grinding with high throughput.
Q. What kind of samples can be grinded?
A.
a) It is suitable for grinding and crushing all kinds of plant tissues,including roots,stems,leaves,
b) It is suitable for grinding and crushingall kinds of animal tissues,including brain,heart,lung,
c) It is suitable for grinding and crushing of food and drug composition analysis and detectionflowers,fruits,seeds and other samplesstomach,liver,thymus,kidney,intestine,lymph node,muscle,bone and other samples
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