DMSO (Dimethyl Sulfoxide) Cell Culture Grade for Scientific Research

Product Information：

DMSO is dimethyl sulfoxide. The molecular formula is C2H6SO, the molecular weight is 78.13, and the purity is > 99.9%. It is sterile and can be used for cell freezing and dissolution of some drugs.

Storage Conditions：

Transport at room temperature, store at room temperature, and the validity period is 36 months.

Instructions for Use:

The usage and dosage of biochemical reagents are mainly based on the customer's experimental purpose and the experimental methods in the existing literature or books, and the following uses are for reference only.

1. Non-proton polar solvent, commonly used as a co-solvent for chemical reactions, PCR reactions, especially ultra-long PCR reactions, to improve the yield, the general concentration of 50-100 mL/L;

2. It can be used as cryoprotectant for cells, tissues and organs, and is used for the freezing of primary and passive cultured cells, recombinant cells, hybridoma cells, embryonic stem cells, hematopoietic stem cells, and the concentration of DMSO in cell freezing is generally 10%;

3. For use in cell culture assays, DMSO is generally used as a solvent for drugs requiring a concentration of no more than 0.1% for toxicity. A final concentration of 0.5% DMSO in cell culture does not cause cytotoxicity. A concentration of 0.1% DMSO is safe for almost all cells;

4. For animal experiments, the mother liquor prepared with DMSO can be diluted with saline or PBS. In order to minimize the toxicity to animals, it is recommended that the final concentration of DMSO should not exceed 2%-5%, and the dosing volume should not be more than 5-10 ml/kg body weight. If the product is not water-soluble, resulting in the precipitation of DMSO mother liquor during dilution, it can be dissolved by adding co-solvents, such as corn oil, Tween 80, glycerol, sodium carboxymethylcellulose and PEG400, etc. It is generally accepted that suspensions are used for oral and intraperitoneal administration without affecting product activity;

5. DMSO was used as a component of the buffer for the following experiments: purification of Poly(A+) RNA, E. coli receptor cell transformation, PCR reactions, amplification of cDNA libraries, DNA sequencing, DEAE-dextran-mediated cell transfection, and polyglutamine-mediated DNA transfection;

6. For mass spectrometry, e.g., using phosphoserine and phosphothreonine residues in DMSO-modified proteins as a phosphorylation state for mass spectrometry (MS).

Notice: 

1. It is easy to overcooling and slowly melt at room temperature. The cured product can be liquefied again at room temperature, which has no effect on the product itself.

2. For Research Use Only.

3. For your safety and health, please wear experimental clothes and disposable gloves.